透皮增强肽介导的表皮生长因子调控小鼠毛发生长的研究

doi:10.3969/j.issn.0253-2778.2018.11.011

中国科学技术大学学报 ›› 2018, Vol. 48 ›› Issue (11): 949-954.DOI: 10.3969/j.issn.0253-2778.2018.11.011

• 论著 • 上一篇

透皮增强肽介导的表皮生长因子调控小鼠毛发生长的研究

金佩佩，王岩石，周馨，叶书来，汪莹，万昕

1.中国科学技术大学附属第一医院（安徽省立医院）检验科，安徽合肥 230036；2.中国科学技术大学附属第一医院（安徽省立医院）妇产科生殖中心，安徽合肥 230036；3.中国科学技术大学附属第一医院（安徽省立医院），安徽省脑立体定向神经外科研究所，安徽合肥 230036

收稿日期:2018-09-20 修回日期:2018-10-20 接受日期:2018-10-20 出版日期:2018-11-30 发布日期:2018-10-20
通讯作者: 万昕
作者简介:金佩佩，女，1988年生，博士.研究方向：细胞生物学.E-mail：kaixin@mail.ustc.edu.cn
基金资助:
国家自然科学基金青年科学基金项目（81701823,31800927）,国家重点研发计划(2018YFC0831101)资助.

Transdermal delivery peptide promoted the regulation of mice hair growth by epidermal growth factor

JIN Peipei, WANG Yanshi, ZHOU Xin, YE Shulai, WANG Ying, WAN Xin

1. Department of Laboratory Medicine, The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Hefei 230036， China; 2. Reproductive Medicine Center, The First Affiliated Hospital of USTC (Anhui Provincial Hospital), Hefei 230036， China; 3. The First Affiliated Hospital of USTC (Anhui Provincial Hospital），Anhui Provincial Stereotactic Neurosurgical Institute， Hefei 230036， China

Received:2018-09-20 Revised:2018-10-20 Accepted:2018-10-20 Online:2018-11-30 Published:2018-10-20

摘要/Abstract

摘要： TD1是一个具有11个氨基酸的短肽（ACSSSPSKHCG），能够促进蛋白药物的透皮转运进而发挥其生物学效应.前期研究显示，利用分子生物学方法得到的TD1-hEGF（T-E）融合蛋白与普通的hEGF相比，其透过完整动物皮肤的效率可提高16倍；基于蛋白的透皮效率具有TD1浓度依赖性的特点设计合成的二代TD1-hEGF:TD1-hEGF-TD1 (T-E-T)及 TD1-TD1-hEGF (2T-E),具有更强的透皮效率.为进一步检测TD1介导的透皮hEGF的生物学效应，我们进行了小鼠毛发生长实验.研究发现T-E,T-E-T和2T-E这三种融合蛋白均可不同程度地诱导小鼠毛囊由休止期转为生长期，调控小鼠毛发的生长.以上结果为透皮增强肽在医学及美容等领域的应用提供了理论基础.

关键词: 透皮给药, 表皮生长因子, 毛发生长周期

Abstract: TD1 was known as a peptide chaperon with 11 amino acids (ACSSSPSKHCG) that can facilitate transdermal delivery of proteins. Previous studies showed the maximum transdermal efficiency of fusion protein TD1-hEGF（T-E） was 16 folds higher than that of native hEGF. The second-generation of TD1-hEGF fused protein: TD1-hEGF-TD1(T-E-T) and TD1-TD1-hEGF (2T-E), designed based on the fact that transdermal efficiency was concentration-dependent of TD1, possessed considerable higher transdermal abilities than TD1-hEGF. To further detect the bioactivity of transdermal hEGF mediated by TD1, a hair growth experiments in mice was conducted. It was found that all the three fused proteins above can induce the transition of hair follicles from the telogen phase to the anagen phase of the hair cycle. These findings provide convincing evidences for improved practicability of the transdermal delivery peptide TD1 in cosmetic.

Key words: transdermal drug delivery, human epidermal growth factor（hEGF）, hair growth cycle

中图分类号:

R319

金佩佩，王岩石，周馨，叶书来，汪莹，万昕. 透皮增强肽介导的表皮生长因子调控小鼠毛发生长的研究[J]. 中国科学技术大学学报, 2018, 48(11): 949-954.

JIN Peipei, WANG Yanshi, ZHOU Xin, YE Shulai, WANG Ying, WAN Xin. Transdermal delivery peptide promoted the regulation of mice hair growth by epidermal growth factor[J]. Journal of University of Science and Technology of China, 2018, 48(11): 949-954.

参考文献

［1］
KURMI B D, TEKCHANDANI P, PALIWAL R, et al. Transdermal drug delivery: Opportunities and challenges for controlled delivery of therapeutic agents using nanocarriers [J]. Current Drug Metabolism, 2017, 18: 481-95.
[2] IBRAHIM Z A, HASSAN G F, ELGENDY H Y, et al. Evaluation of the efficacy of transdermal drug delivery of calcipotriol plus betamethasone versus tacrolimus in the treatment of vitiligo [J]. Journal of Cosmetic Dermatology, 2018: DOI=10.1111/jocd.12704.
[3] AMMAR H O, GHORAB M, EL-NAHHAS S A, et al. Design of a transdermal delivery system for aspirin as an antithrombotic drug [J]. International Journal of Pharmaceutics, 2006, 327: 81-88.
[4] SIXEL-DORING F, TRENKWALDER C. Rotigotine transdermal delivery for the treatment of restless legs syndrome [J]. Expert Opinionon Pharmacotherapy, 2010,11: 649-656.
[5] JIANG T, WANG T, LI T, et al. Enhanced transdermal drug delivery by transfersome-embedded oligopeptide hydrogel for topical chemotherapy of melanoma [J]. ACS NANO, 2018, 12 (10): 9693-9701.
[6] BROWN M B, MARTIN G P, JONES S A, et al. Dermal and transdermal drug delivery systems: Current and future prospects [J]. Drug Delivery, 2006, 13: 175-187.
[7] COURTENAY A J, MCCRUDDEN M T C, MCAVOY K J, et al. Microneedle-mediated transdermal delivery of bevacizumab[J]. Molecular Pharmaceutics [J]. 2018,15: 3545-3556.
[8] CEFALU W T. Concept, strategies, and feasibility of noninvasive insulin delivery [J]. Diabetes Care, 2004, 27: 239-246.
[9] CHEN Y, SHEN Y, GUO X, et al. Transdermal protein delivery by a coadministered peptide identified via phage display [J]. Nature Biotechnology, 2006, 24: 455-460.
[10] RUAN R Q, WANG S S, WANG C L, et al. Transdermal delivery of human epidermal growth factor facilitated by a peptide chaperon [J]. European Journal of Medicinal Chemistry, 2013, 62: 405-409.
[11] JIN P P, LI F F, RUAN R Q, et al. Enhanced transdermal delivery of epidermal growth factor facilitated by dual peptide chaperone motifs [J]. Protein and Peptide Letters, 2014, 21: 550-555.
[12] HERMAN A, HERMAN A P. Mechanism of action of herbs and their active constituents used in hair loss treatment [J]. Fitoterapia, 2016, 114: 18-25.
[13] WU Z, SUN L, LIU G, et al. Hair follicle development and related gene and protein expression of skins in Rex rabbits during the first 8 weeks of life [J]. Asian-Australasian Journal of Animal Sciences, 2018: DOI= 10.5713/ajas.18.0256.
[14] BICHSEL K J, GOGIA N, MALOUFF T, et al. Role for the epidermal growth factor receptor in chemotherapy-induced alopecia [J]. PLoS ONE, 2013, 8(7): e69368.
[15] PAIK S H, YOON J S, RYU H H, et al. Pretreatment of epidermal growth factor promotes primary hair recovery via the dystrophic anagen pathway after chemotherapy-induced alopecia[J]. Experimental Dermatology, 2013, 22(7): 496-499.
[16] WEE P, WANG Z. Epidermal growth factor receptor cell proliferation signaling pathways [J]. Cancers, 2017, 9(5): 52.
[17] HUYNH E, LI J. Generation of Lactococcus lactis capable of coexpressing epidermal growth factor and trefoil factor to enhance in vitro wound healing [J]. Applied Microbiology and Biotechnology, 2015, 99(11): 4667-4677.
[18] WALTER M N, WRIGHT K T, FULLER H R, et al. Mesenchymal stem cell-conditioned medium accelerates skin wound healing: An in vitro study of fibroblast and keratinocyte scratch assays [J]. Experimental Cell Research, 2010, 316(7): 1271-1281.
[19] LUSK J B, LAM V Y, TOLWINSKI N S. Epidermal growth factor pathway signaling in drosophila embryogenesis: Tools for understanding cancer [J]. Cancers, 2017, 9(2): 16.
[20] XING X J, YANG L, YOU Y, et al. Study of the biological function and penetration pathways of the mouse epidermal growth factor ethosomal delivery system [J]. Experimental Dermatology, 2011, 20(11): 945-947.

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透皮增强肽介导的表皮生长因子调控小鼠毛发生长的研究

Transdermal delivery peptide promoted the regulation of mice hair growth by epidermal growth factor

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