棉铃虫滞育解除的相关基因鉴定

doi:10.3969/j.issn.0253-2778.2010.01.008

中国科学技术大学学报 ›› 2010, Vol. 40 ›› Issue (1): 48-52.DOI: 10.3969/j.issn.0253-2778.2010.01.008

棉铃虫滞育解除的相关基因鉴定

朱佳

1.中国科学技术大学生命科学学院，安徽合肥 230027; 2.中山大学生命科学学院，有害生物控制与资源利用国家重点实验室，广东广州 510275

收稿日期:2009-03-24 修回日期:2009-04-22 出版日期:2010-01-31 发布日期:2010-01-31
通讯作者: 徐卫华
作者简介:朱佳，男，1981年生，硕士. 研究方向：昆虫遗传学. E-mail: zhuj@mail.ustc.edu.cn
基金资助:
国家自然科学基金（30730014）资助.

Identification of the related genes at diapause termination in Helicoverpa armigera

ZHU Jia

1.School of Life Sciences, University of Science and Technology of China, Hefei 230027,China; 2.State Key Laboratory of Biocontrol, and School of Life Sciences, Sun Yat-Sen (Zhongshan) University, Guangzhou 510275, China

Received:2009-03-24 Revised:2009-04-22 Online:2010-01-31 Published:2010-01-31

摘要/Abstract

摘要： 运用差异显示PCR(DD-PCR)方法分析棉铃虫滞育解除蛹差异表达的基因，结果得到了56个差异片段.通过RT-PCR和Real-time PCR的方法，鉴定了滞育解除过程中有3个基因表达量在下调，有4个基因表达量在上调.这7个差异表达基因中有3个和已知的基因有较高的同源性：FKBP12，esr16和NADH dehydrogenase 1 alpha subcomplex subunit 6.这些差异基因为今后研究滞育解除的分子机制提供了新的线索.

关键词: 滞育, 20-羟基蜕皮酮, 差异显示PCR, 棉铃虫

Abstract: By means of mRNA differential display PCR(DD-PCR), 56 cDNA fragments have been identified from the Helicoverpa armigeras brain that are expressed differentially between the diapausing pupae and the pupae of diapause termination by injecting 20-hydroxyecdysone. RT-PCR and Real-time PCR analysis revealed that mRNA levels of the FK506-binding protein 12(FKBP12)and two unknown genes were higher in the diapausing pupae, whereas the ecdysteroid-regulated 16 kDa protein(esr16), NADH dehydrogenase 1 alpha subcomplex subunit 6, and two unknown genes were higher in the pupae of diapause termination. These results provide new clues to researches on the molecular mechanism of diapause.

Key words: diapause, 20-hydroxyecdysone, differential display PCR, Helicoverpa armigera

朱佳，杨靖，徐卫华. 棉铃虫滞育解除的相关基因鉴定[J]. 中国科学技术大学学报, 2010, 40(1): 48-52.

ZHU Jia, YANG Jing, XU Weihua. Identification of the related genes at diapause termination in Helicoverpa armigera[J]. Journal of University of Science and Technology of China, 2010, 40(1): 48-52.

棉铃虫滞育解除的相关基因鉴定

Identification of the related genes at diapause termination in Helicoverpa armigera

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