持续黑暗状态下对大鼠松果体细胞分泌松果体素的昼夜节律研究

doi:10.3969/j.issn.0253-2778.2011.01.005

中国科学技术大学学报 ›› 2011, Vol. 41 ›› Issue (1): 29-34.DOI: 10.3969/j.issn.0253-2778.2011.01.005

• 原创论文 • 上一篇

持续黑暗状态下对大鼠松果体细胞分泌松果体素的昼夜节律研究

刘雅静

1.中国科学技术大学生命科学学院，安徽合肥 230027； 2.上海大学生命科学学院，上海 200444；3.杭州市第七人民医院，浙江杭州 310013

收稿日期:2010-06-23 修回日期:2011-01-15 出版日期:2011-01-31 发布日期:2011-01-31

Circadian rhythms in rat pineal melatonin synthesis under constant darkness conditions

LIU Yajing

1.Hefei National Laboratory for Physical Sciences at Microscale, and School of Life Science, University of Science and Technology of China, Hefei 230027, China； 2.School of Life Science, Shanghai University, Shanghai 200444, China； 3.The Seventh Hospital of Hangzhou, Hangzhou 310013, China

Received:2010-06-23 Revised:2011-01-15 Online:2011-01-31 Published:2011-01-31
Contact: ZHOU Jiangning
About author:LIU Yajing, female, born in 1978, PhD. Research field: neurodegenerative disease. E-mail: yjl@ustc.edu.cn

摘要/Abstract

摘要： 为研究体外培养的大鼠松果体细胞分泌的松果体素是否具有内在节律，收集体外培养的大鼠松果体细胞在白天6∶00,8∶00,10∶00,12∶00,15∶00, 和夜晚18∶00,20∶00,22∶00,1∶00,4∶00各个时间点的培养上清，并用放射免疫方法（RIA）检测各个时间点松果体细胞培养上清中的松果体素水平.CCF（complex cosine function）统计分析结果表明，在持续黑暗的环境下，体外培养的大鼠松果体细胞分泌的松果体素在培养3，6，9天后仍然表现出内在节律.然而，这种内在节律在培养12天后消失.上述结果提示体外培养的大鼠松果体细胞可以作为一个有用的体外模型来分析节律系统的生理基础.

关键词: 大鼠, 松果体, 细胞培养, 松果体素, 持续黑暗的环境, 节律

Abstract: To investigate whether rat pineal cell is capable of generating a circadian oscillation in melatonin release，the cultured pineal cell in culture medium was collected at 6∶00, 8∶00, 10∶00, 12∶00 and 15∶00 during the day, and at 18∶00, 20∶00, 22∶00, 1∶00 and 4∶00 at night respectively. The melatonin level in culture medium was measured by a direct radioimmunoassay. The complex cosine functions (CCF) statistical analysis indicates that the rat pineal cell melatonin synthesis shows an intrinsic rhythm at 3, 6, 9 day culture under constant dark conditions. However, the intrinsic rhythm disappears at 12 day culture. This result suggests that the rat individual pineal cells can function as a circadian oscillator in vitro.

Key words: rat, pineal, cell culture, melatonin, constant darkness, circadian

刘雅静，陆仲敏，朱红艳，谭忠林，周江宁. 持续黑暗状态下对大鼠松果体细胞分泌松果体素的昼夜节律研究[J]. 中国科学技术大学学报, 2011, 41(1): 29-34.

LIU Yajing, LU Zhongmin, ZHU Hongyan, TAN Zhonglin, ZHOU Jiangning. Circadian rhythms in rat pineal melatonin synthesis under constant darkness conditions[J]. Journal of University of Science and Technology of China, 2011, 41(1): 29-34.

持续黑暗状态下对大鼠松果体细胞分泌松果体素的昼夜节律研究

Circadian rhythms in rat pineal melatonin synthesis under constant darkness conditions

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