一套用于秀丽隐杆线虫多感觉信息整合机制研究的行为学系统

doi:10.52396/JUST-2021-0096

摘要/Abstract

摘要： 动物通过感知不同的感觉信息来介导自己的行为.神经系统如何进行多感觉信息整合仍然是神经科学领域一个悬而未决的问题.本文对秀丽隐杆线虫如何整合温度和盐刺激这两种对其生存至关重要的刺激进行研究.我们设计了一套行为学系统,可以在正交方向同时产生稳定的线性温度和盐浓度梯度.通过将本文中的行为学系统与一套追踪系统和钙成像系统结合,我们对秀丽隐杆线虫趋向行为的行为策略以及参与这一行为的两个主要感觉神经元 AFD 和 ASER 的功能进行分析.我们的工作开启了一个全新的视野,在精巧的神经系统结构中进行多感觉信息整合的研究.

关键词: 行为学系统, 温度梯度, 盐梯度, 多感觉信息整合

Abstract: Animals use different sensory cues to guide their behaviors. How a nervous system performs multi-sensory integration remains an open question in neuroscience. Here, we study how the nematode Caenorhabditis elegans integrates temperature and salt stimuli, two environmental cues that are essential for survival. We report the development of a navigational system that allows stable, linear, and orthogonal temperature gradient and salt gradient to be simultaneously presented to an animal. By combining this setup with a tracking and calcium imaging system, we analyze the behavioral strategies of C. elegans and investigate the functions of two cilia sensory neurons—AFD and ASER—that attend the behavior. Our work opens a new window into interrogating the neural mechanisms for multi-sensory integration in a compact nervous system.

Key words: behavioral system, temperature gradient, salt gradient, multi-sensory integration

中图分类号:

赵秉珍, 罗林娇, 温泉. 一套用于秀丽隐杆线虫多感觉信息整合机制研究的行为学系统[J]. 中国科学技术大学学报, 2021, 51(5): 353-359.

ZHAO Bingzhen, LUO Linjiao, WEN Quan. A navigational system for investigating multi-sensory integration in Caenorhabditis elegans[J]. Journal of University of Science and Technology of China, 2021, 51(5): 353-359.

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