植物细胞壁物质在DMSO-d6/HMPA-d18中的溶液态2D HSQC NMR分析

doi:10.3969/j.issn.0253-2778.2020.08.006

中国科学技术大学学报 ›› 2020, Vol. 50 ›› Issue (8): 1077-1083.DOI: 10.3969/j.issn.0253-2778.2020.08.006

植物细胞壁物质在DMSO-d6/HMPA-d18中的溶液态2D HSQC NMR分析

王婉婉，蔡继宝，徐振宇，牛凡超，苏加坤，张翼，杨俊*

1．中国科学技术大学化学系，安徽合肥 230026;2．江西中烟工业有限责任公司技术中心，江西南昌 330096

收稿日期:2020-06-06 修回日期:2020-06-28 接受日期:2020-06-28 出版日期:2020-08-31 发布日期:2020-06-28
通讯作者: 杨俊
作者简介:王婉婉,女, 1994年生,硕士.研究方向: 分析化学. E-mail: sa170191@mail.ustc.edu.cn
基金资助:
江西中烟工业有限责任公司科技项目( 2020-016)资助．

2D HSQC NMR analysis of plant cell wall material in DMSO-d6/HMPA-d18

WANG Wanwan, CAI Jibao, XU Zhenyu, NIU Fanchao, SU Jiakun, ZHANG Yi, YANG Jun*

1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China; 2. Technology Center, China Tobacco Jiangxi Industry Co., Ltd., Nanchang 330096, China

Received:2020-06-06 Revised:2020-06-28 Accepted:2020-06-28 Online:2020-08-31 Published:2020-06-28

摘要/Abstract

摘要： 细胞壁物质凝胶是细胞壁物质直接在核磁共振管中与氘代溶剂生成的产物，无须分离成分就可以获得细胞壁良好分辨的2D 13C-1H相关核磁共振谱（2D HSQC NMR）.为此选用氘代二甲基亚砜（DMSO-d6）、氘代二甲基亚砜/氘代吡啶（DMSO-d6/ pyridine-d5）和氘代二甲基亚砜/氘代六甲基磷酰三胺（DMSO-d6/HMPA-d18）这3种溶剂溶解白杨（被子植物）细胞壁，DMSO-d6/HMPA-d18下得到的图谱信号最丰富，且很容易获得羟基苯基相关信号，所以DMSO-d6/ HMPA-d18提高了图谱的分辨率和强度.将DMSO-d6/HMPA-d18应用于松树（裸子植物）的二维核磁结构表征中，图谱显示了高分辨率的多糖相关性和木质素结构.该法适用于植物的细胞壁物质检测，因此这种DMSO-d6/HMPA-d18下的2D HSQC NMR研究是一种更快速,绿色的植物细胞壁结构的评估方法.

关键词: 凝胶态, 细胞壁物质, 2D HSQC NMR, DMSO-d6/HMPA-d18

Abstract: The cell wall material gel is the product of the cell wall material directly formed with deuterated solvent in the nuclear magnetic resonance tube. The well-resolved/dispersed 2D 13C-1H related nuclear magnetic resonance spectra (2D HSQC NMR)can be obtained without the separation of components. Deuterated dimethyl sulfoxide (DMSO-d6), deuterated dimethyl sulfoxide/deuterated pyridine (DMSO-d6/pyridine-d5) and deuterated dimethyl sulfoxide/deuterated hexamethylphosphoryltriamine (DMSO-d6/HMPA-d18) were selected to dissolve the cell wall of poplar (angiosperm). The spectrum signals under DMSO-d6/HMPA-d18 were the most abundant, and the correlation of p-hydroxyphenyl signals could be easily obtained. Therefore, DMSO-d6/ HMPA-d18 improves the resolution and intensity of the spectrum. DMSO-d6/HMPA-d18 was used to characterize the two-dimensional NMR structure of pine (gymnosperms), and the spectrum showed high-resolution polysaccharide correlation and lignin structure. Studies have shown that this method is suitable for the detection of cell wall substances in plants.Therefore, the 2D HSQC NMR study under DMSO-d6/HMPA-d18 is a faster and greener method to evaluate the structure of plant cell wall.

Key words: Gel state, cell wall substance, 2D HSQC NMR, DMSO-d6/HMPA-d18

中图分类号:

O656.4

王婉婉，蔡继宝，徐振宇，牛凡超，苏加坤，张翼，杨俊*. 植物细胞壁物质在DMSO-d6/HMPA-d18中的溶液态2D HSQC NMR分析[J]. 中国科学技术大学学报, 2020, 50(8): 1077-1083.

WANG Wanwan, CAI Jibao, XU Zhenyu, NIU Fanchao, SU Jiakun, ZHANG Yi, YANG Jun*. 2D HSQC NMR analysis of plant cell wall material in DMSO-d6/HMPA-d18[J]. Journal of University of Science and Technology of China, 2020, 50(8): 1077-1083.

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植物细胞壁物质在DMSO-d6/HMPA-d18中的溶液态2D HSQC NMR分析

2D HSQC NMR analysis of plant cell wall material in DMSO-d6/HMPA-d18

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