Abstract: TD1 was known as a peptide chaperon with 11 amino acids (ACSSSPSKHCG) that can facilitate transdermal delivery of proteins. Previous studies showed the maximum transdermal efficiency of fusion protein TD1-hEGF（T-E） was 16 folds higher than that of native hEGF. The second-generation of TD1-hEGF fused protein: TD1-hEGF-TD1(T-E-T) and TD1-TD1-hEGF (2T-E), designed based on the fact that transdermal efficiency was concentration-dependent of TD1, possessed considerable higher transdermal abilities than TD1-hEGF. To further detect the bioactivity of transdermal hEGF mediated by TD1, a hair growth experiments in mice was conducted. It was found that all the three fused proteins above can induce the transition of hair follicles from the telogen phase to the anagen phase of the hair cycle. These findings provide convincing evidences for improved practicability of the transdermal delivery peptide TD1 in cosmetic.

Key words: transdermal drug delivery, human epidermal growth factor（hEGF）, hair growth cycle