Journal of University of Science and Technology of China ›› 2020, Vol. 50 ›› Issue (8): 1077-1083.DOI: 10.3969/j.issn.0253-2778.2020.08.006

• Original Paper • Previous Articles     Next Articles

2D HSQC NMR analysis of plant cell wall material in DMSO-d6/HMPA-d18

WANG Wanwan, CAI Jibao, XU Zhenyu, NIU Fanchao, SU Jiakun, ZHANG Yi, YANG Jun*   

  1. 1. Department of Chemistry, University of Science and Technology of China, Hefei 230026, China; 2. Technology Center, China Tobacco Jiangxi Industry Co., Ltd., Nanchang 330096, China
  • Received:2020-06-06 Revised:2020-06-28 Accepted:2020-06-28 Online:2020-08-31 Published:2020-06-28

Abstract: The cell wall material gel is the product of the cell wall material directly formed with deuterated solvent in the nuclear magnetic resonance tube. The well-resolved/dispersed 2D 13C-1H related nuclear magnetic resonance spectra (2D HSQC NMR)can be obtained without the separation of components. Deuterated dimethyl sulfoxide (DMSO-d6), deuterated dimethyl sulfoxide/deuterated pyridine (DMSO-d6/pyridine-d5) and deuterated dimethyl sulfoxide/deuterated hexamethylphosphoryltriamine (DMSO-d6/HMPA-d18) were selected to dissolve the cell wall of poplar (angiosperm). The spectrum signals under DMSO-d6/HMPA-d18 were the most abundant, and the correlation of p-hydroxyphenyl signals could be easily obtained. Therefore, DMSO-d6/ HMPA-d18 improves the resolution and intensity of the spectrum. DMSO-d6/HMPA-d18 was used to characterize the two-dimensional NMR structure of pine (gymnosperms), and the spectrum showed high-resolution polysaccharide correlation and lignin structure. Studies have shown that this method is suitable for the detection of cell wall substances in plants.Therefore, the 2D HSQC NMR study under DMSO-d6/HMPA-d18 is a faster and greener method to evaluate the structure of plant cell wall.

Key words: Gel state, cell wall substance, 2D HSQC NMR, DMSO-d6/HMPA-d18

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