DNA损伤环境下的裂殖酵母lncRNA表达谱分析

doi:10.52396/JUST-2021-0074

摘要/Abstract

摘要： LncRNA在真核生物中广泛转录,且响应于细胞外环境的变化.相比于mRNA,人们对lncRNA在DNA损伤应答中的作用了解甚少.基于高通量测序,本文系统地分析了裂殖酵母在4种DNA损伤药物(喜树碱、羟基脲、甲基磺酸甲酯和腐草霉素)处理下的lncRNA表达谱.与mRNA相似,在DNA损伤环境下,lncRNA的表达谱也发生了剧烈变化.161个受到4种药物共同诱导的lncRNA及194个受到共同抑制的lncRNA被定义为核心DNA损伤应答lncRNA.LncRNA表达谱和mRNA表达谱之间的差异表明,lncRNA在DNA损伤应答中起着重要功能,且这些功能可能并不依赖于调控邻近的mRNA.这项研究为进一步研究裂殖酵母中的lncRNA在DNA损伤应答中的功能提供了基础和参考.

关键词: 长链非编码RNA, DNA损伤, 裂殖酵母

Abstract: LncRNAs are pervasively transcribed in eukaryotic cells and dynamically regulated in response to environmental changes. Compared to mRNAs, roles of lncRNAs in DNA damage responses are poorly understood. The lncRNA expression profiles of Schizosaccharomyces pombe under the treatment of four kinds of DNA damage generating drugs (camptothecin, hydroxyurea, methyl methanesulfonate and phleomycin) were systematically characterized by RNA-seq. Similar to mRNAs, lncRNA repertoires underwent drastic changes in response to DNA damage inducing environments. A Core DNA Damage Response lncRNA set of 161 commonly induced and 194 commonly repressed lncRNAs was identified. The differences between lncRNA and mRNA transcription profiles suggested that lncRNAs might conduct critical functions in DNA damage responses independent from their associated mRNAs. This profiling on lncRNA expressions provided data and resources for further functional studies of the fission yeast lncRNAs.

Key words: long noncoding RNA, DNA damage, Schizosaccharomyces pombe

中图分类号:

于添翼, 武瑞堃, 单革, 任冰冰. DNA损伤环境下的裂殖酵母lncRNA表达谱分析[J]. 中国科学技术大学学报, 2021, 51(8): 586-597.

YU Tianyi, WU Ruikun, SHAN Ge, REN Bingbing. LncRNA expression profiles of Schizosaccharomyces pombe in DNA damage inducing environments[J]. Journal of University of Science and Technology of China, 2021, 51(8): 586-597.

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