Abstract: To research whether there is any change of ASPP (Apoptosis-stimulating protein of p53) family, including ASPP1, ASPP2 and iASPP, during the process of early development of the Rhesus neural system, and to research the tendency of the change primarily, the process of early development of Rhesus neural system was simulated through induced neural differentiation from rESC (Rhesus embryonic stem cells) in vitro; meanwhile, the changes of ASPP family in cells were detected by Immunohistochemical Staining and western blotting. The results show that changes of ASPP family in cells have been detected in the process of induced neural differentiation from rESC (Rhesus embryonic stem cells) in vitro, especially amounts and modifications, and some tendencies of these changes can be indicated from these results. The results suggest that there is some certain connection between ASPP family and early development of Rhesus neural system. It might be helpful for diagnosis and treatment of neuronal degenerative diseases and tumours with neuronal origin in the future.

Key words: ASPP1, ASPP2, iASPP, neural progenitors, Rhesus embryonic stem cells