Journal of University of Science and Technology of China ›› 2019, Vol. 49 ›› Issue (4): 297-301.DOI: 10.3969/j.issn.0253-2778.2019.04.006

• Original Paper • Previous Articles     Next Articles

Nanobody cluster imaging probe based on sortase and transglutaminase

TIAN Yao, WU Tiantian, LIU Yangzhong   

  1. School of Chemistry and Materials Science, University of Science and Technology of China, Hefei 230026, China
  • Received:2018-04-12 Revised:2018-09-14 Accepted:2018-09-14 Online:2019-04-30 Published:2018-09-14

Abstract: Sortase and transglutaminase was rendered for preparing a targeted protein cluster probe, which is composed nanobody and green fluorescence protein (sfGFP). A unique substrate, which can be recognized by both sortase and transglutaminase, was designed for nanobody and sfGFP conjunction by solid phase synthesis. The targeted protein cluster probe, namely KK-sfGFP(Nb), can specificly bind to the EGFR overexpressed cells and shows green fluorescence as signal. The KK-sfGFP(Nb) exhibits fast accumulation in mouse xenograft tumor and the fluorescence signal gradually metabolizes within 9 h.

Key words: sortase, transglutaminase, nanobody, site-specific modification, target imaging

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