表面修饰的金纳米棒的生物相容性和细胞摄取研究

doi:10.3969/j.issn.0253-2778.2011.05.008

中国科学技术大学学报 ›› 2011, Vol. 41 ›› Issue (5): 428-431.DOI: 10.3969/j.issn.0253-2778.2011.05.008

表面修饰的金纳米棒的生物相容性和细胞摄取研究

方文彦

1.淮南师范学院化学与化工系，安徽淮南 232001；2.中国科学技术大学化学与材料科学学院，安徽合肥 230026

收稿日期:2010-11-11 修回日期:2011-04-22 出版日期:2011-05-31 发布日期:2011-05-31
通讯作者: 方文彦
作者简介:方文彦(通讯作者),女,1963年生，硕士/副教授. 研究方向:应用化学. E-mail：wyfang6312@126.com
基金资助:
安徽省高校省级自然科学重点研究项目(KJ2009A008)，淮南市科技计划项目(2010A03205)资助.

Biocompatibility and cellular uptake of surface-modified gold nanorods

FANG Wenyan

1.Department of Chemistry and Chemical Engineering, Huainan Normal University, Huainan 232001, China; 2.School of Chemistry and Materials, University of Science and Technology of China, Hefei 230026, China

Received:2010-11-11 Revised:2011-04-22 Online:2011-05-31 Published:2011-05-31

摘要/Abstract

摘要： 制备了棒状纳米金颗粒并对其表面进行了修饰，研究了其生物相容性以及运载荧光物质进入细胞的功能.所制备的材料通过聚乙二醇稳定、采用双修饰的方法获得了具有活性-NH2基团存在、稳定性好且生物相容性高的表面修饰的金纳米棒.采用透射电子显微镜和紫外可见吸收光谱对表面修饰的金纳米棒进行了表征，运用MTT比色分析法通过细胞毒性研究了表面修饰的金纳米棒的生物相容性，用流式细胞仪和激光共聚焦显微镜考察了其运载荧光物质进入细胞的功能.结果表明，表面修饰的金纳米棒具有良好的生物相容性，具有作为荧光物质或药物载体的应用前景，可应用于疾病的诊断治疗.

关键词: 金纳米棒, 表面修饰, 聚乙二醇, 荧光探针

Abstract: Surface modified gold nanorods which are hexadecyltrimethylammonium bromide (CTAB) stabilized were obtained and their biocompatibility and function of delivering fluorescent molecules into cells were also studied. The CTAB stabilized gold nanorods were surface modified with polyethylene glycol (PEG) through in situ dithiocarbamate formation and ligand exchange. The surface modified gold naorods (PEG-GNRs) with terminal amine on its surface was prepared. The shape and optical properties of surface modified GNRs were characterized by transmission electron microscope (TEM) and UV-Vis. Then, the biocompatibility of PEG-GNRs was measured with MTT assay. A confocal laser scanning microscope (CLSM) and a fluorescence activated cell sorter (FACS) were employed to study PEG-GNRs function of delivering fluorescence molecules into cells. Results show that PEG-GNRs have good compatibility and can be used as a vector for delivering fluorescent molecules or drug into cells for cell imaging in the future.

Key words: gold nanorods, surface modification, polyethylene glycol (PEG), fluorescent probe

方文彦，闵元增，徐迈，朱传高. 表面修饰的金纳米棒的生物相容性和细胞摄取研究[J]. 中国科学技术大学学报, 2011, 41(5): 428-431.

FANG Wenyan, MIN Yuanzeng, XU Mai, ZHU Chuangao. Biocompatibility and cellular uptake of surface-modified gold nanorods[J]. Journal of University of Science and Technology of China, 2011, 41(5): 428-431.

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表面修饰的金纳米棒的生物相容性和细胞摄取研究

Biocompatibility and cellular uptake of surface-modified gold nanorods

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