基于电沉积石墨烯/普鲁士蓝/壳聚糖复合薄膜的葡萄糖生物传感器

doi:10.3969/j.issn.0253-2778.2019.04.002

中国科学技术大学学报 ›› 2019, Vol. 49 ›› Issue (4): 268-275.DOI: 10.3969/j.issn.0253-2778.2019.04.002

基于电沉积石墨烯/普鲁士蓝/壳聚糖复合薄膜的葡萄糖生物传感器

刘皓，吴守国

1.常州卫生高等职业技术学校，江苏常州 213002;2.中国科学技术大学化学系，安徽合肥 230026

收稿日期:2018-10-10 修回日期:2018-12-18 接受日期:2018-12-18 出版日期:2019-04-30 发布日期:2018-12-18

A glucose biosensor based on graphene-Prussian blue-chitosan composite film fabricated by electrodeposition

LIU Hao, WU Shouguo2

Received:2018-10-10 Revised:2018-12-18 Accepted:2018-12-18 Online:2019-04-30 Published:2018-12-18
Contact: WU Shouguo
About author:LIU Hao, male, born in 1988, master. Research field: electroanalytical chemistry. E-mail: hao0925@mail.ustc.edu.cn

摘要/Abstract

摘要： 在玻碳电极表面通过三步电沉积法制备了石墨烯/普鲁士蓝/壳聚糖复合薄膜葡萄糖生物传感器.通过循环伏安法将氧化石墨烯电化学还原，在电极表面直接得到石墨烯纳米层，在石墨烯纳米层上成功电沉积得到普鲁士蓝纳米粒子和葡萄糖氧化酶-壳聚糖复合薄膜，制备的修饰电极通过电化学方法以及扫描电镜分析了其性能与结构.在最优条件下，该生物传感器表现出了灵敏度高（50.29 mA· L·mol-1·cm-2）、检测限低（12 μmol·L-1）、响应时间短（3 s）等特点.电极响应电流与葡萄糖溶液浓度在0.02～10 mmol·L-1范围内具有较好的线性关系.此外，在对人体血清样本的检测中，该传感器同样表现出优异的性能，对血清中常见物质具有较强的抗干扰能力.

关键词: 葡萄糖生物传感器, 石墨烯, 普鲁士蓝, 壳聚糖, 电化学沉积

Abstract: The layer-by-layer structured composite film of graphene-Prussian blue (PB)-chitosan (CS) was fabricated on a glassy carbon electrode by three-step electrodeposition method and used for glucose sensing. Graphene nanosheets were directly deposited onto the electrode through reduction of graphene oxide by cyclic potential scanning. Then, a glucose biosensor was fabricated by electrodepositing PB nanoparticles and glucose oxidase (GOD)-chitosan hybrid film (GOD-CS) on the graphene modified electrode successively. The surface of the resulting modified electrode was characterized by electrochemical methods and scanning electron microscopy. Under optimal conditions, the biosensor shows high sensitivity (50.29 mA· L·mol-1·cm-2), low detection limit (12 μmol·L-1) and fast response time (3 s). A linear dependence of the catalytic current upon glucose concentration is obtained in a wide range from 0.02 to 10 mmol·L-1. In addition, the sensor also performs well for measuring glucose concentrations in human blood serum samples without any pretreatment.

Key words: glucose biosensor, graphene, Prussian blue, chitosan, electrochemical deposition

中图分类号:

O657.1

刘皓，吴守国. 基于电沉积石墨烯/普鲁士蓝/壳聚糖复合薄膜的葡萄糖生物传感器[J]. 中国科学技术大学学报, 2019, 49(4): 268-275.

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基于电沉积石墨烯/普鲁士蓝/壳聚糖复合薄膜的葡萄糖生物传感器

A glucose biosensor based on graphene-Prussian blue-chitosan composite film fabricated by electrodeposition

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